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Title: Development of a field test kit for detection of blue-green algal toxins (348/05/A) : interim progress report
Author: National Rivers Authority
Document Type: Monograph
Annotation: EA additional title info: Biocode Limited February 1993 R&D 348/OS/A
During the period November 1992 to January 1993, work has continued on Stage 2 of the original proposal - production and coupling of selected monoclonal antibodies to an inert matrix. In this quarter the NRA has continued to characterise the four antibodies which were selected for their ability to bind to microcystin-LR. Optimisation of the competitive ELISA led to determination of an IC50, of 77 ng/ml for the antibody A005-1A 13C6. However, no IC50 was established for the other three key antibodies (A005-1A 15C4, A005-1D 3D1 and A005-1F 8D2). Consequently, efforts have been concentrated on the application of the A005-1D 13C6 antibody to kit formatting. The A005-1D 13C6 antibody has been coupled to cyanogen bromide and triazine activated supports with the aim of producing immunoaffinity (EASI-EXTRACT) columns for the detection of microcystin-LR. When solutions of microcystin-LR were applied to the immunoaffinity columns, no antigen binding was found. Alternative coupling procedures are being investigated and different kit formats are to be considered. Furthermore, an alternative procedure for the purification of the IgM antibody has been developed.
Publisher: National Rivers Authority
Publication Date: 1993
Publication Place: [Bristol]
Subject Keywords: AntibodiesAlgaeResearch projectsExperimentation
Taxonomic Keywords: Cyanobacteria
Extent: 19
Total file downloads: 263

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