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Title: Development of a field test kit for detection of blue-green algal toxins (348/02/A) : interim progress report
Author: National Rivers Authority
Document Type: Monograph
Work has continued on Stage 1 of this project to develop monoclonal antibodies to microcystin LR. A single mouse was selected for fusion on the basis of the results presented in the previous report. From this, three cultures have been selected that bind to microcystin LR coated plates. The isolation and characterisation of monoclonal cell lines from these cultures is ongoing. Initial difficulties in detecting antibodies that bind to the unconjugated microcystin LR have been overcome by the use of enzyme linked immuno sorbent assay (ELISA) plates coated directly with the unconjugated toxin. The quantity of microcystin LR required for plate coating has been substantially reduced by pretreating the ELISA plates with poly L lysine. Immunisations with the unbridged microcystin LR conjugate have continued. In addition a new batch of bridged conjugate has been prepared and used to immunise a further batch of mice. As a result, another four mice have been selected for fusion. From these four fusions fourteen additional cultures have been isolated that produce antibody to microcystin LR. Monoclonal cell lines are currently being selected from these cultures.
Publisher: National Rivers Authority
Publication Date: 1992
Publication Place: Bristol
Subject Keywords: CyanophyceaeAntibodiesLaboratory experimentationTesting
Extent: 16
Total file downloads: 294

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